Maintaining postharvest quality of fresh bananas is a major trading challenge of the industry. 1-Methylcyclopropene (1-MCP) can prolong postharvest quality of banana fruit by delaying the expression of ripening attributes and senescence through preventing binding of ethylene to its receptors thereby inhibiting ethylene signal transduction and downstream action. Several researches have been conducted to investigate the effect of 1-MCP in bananas, however, inconsistencies of the results have been reported. Moreover, effectiveness of 1-MCP is governed by various factors such as cultivar, fruit maturity, concentration, time of exposure and method of application. In this study, the effect of preharvest methods (Stalk end immersion (SEI), Bunch spraying (BS), Combination (SEI+BS), and Control) of 1-MCP application was determined through observation on the postharvest quality of ‘Cavendish’ bananas such as peel yellowing, sensory firmness, visual quality, weight loss, degree of shrivelling, fruit finger drop, organoleptic attributes, chemical properties (Total Soluble Solids, Total Titratable Acidity and pH) and disease incidence. Cost of each treatment was also determined. Results revealed that the preharvest methods of 1-MCP (aqueous solution dosage of 400 nL L-1) application through SEI and SEI+BS methods significantly retarded the peel color change up to 7 days of storage and prolonged its shelf life for up to 19 days under ambient storage condition. However, SEI+BS delayed fruit softening (for 15 days) and maintained visual quality (for 19 days) compared to SEI. In addition, fruits treated with 1-MCP through SEI+BS have lesser accumulated weight loss, lower degree of shriveling and reduced incidence of finger drop compared to BS and SEI methods. In terms of cost efficiency, BS had lower cost compared to SEI and SEI+BS methods. On the other hand, the chemical properties, organoleptic attributes and disease incidence (crown discoloration, crown rot/mold and banana anthracnose) failed to show any significant difference among preharvest methods.