Using polymer as a mean of irrigation has shown minimal moisture reduction. Thus, the irrigation schedule should be regulated. Also, the polymer has a vital role in producing banana plantlets to mitigate the adverse climate condition. This study determines the effect of polymer irrigation on the growth response of lakatan banana under nursery condition. Three factors were used, Factor 1 irrigation amount (A1=25mm/week, A2=20mm/week, A3= 15 mm/week), Factor 2 irrigation frequency (B1=once a week, B2=twice a week, B3= thrice a week) and Factor 3 amount of polymer (C1= 0 grams and C2=5 grams). The data were analyzed using Analysis of Variance (ANOVA) 3x3x2 Factorial experiment in split-split plot design arranged in Randomized Complete Block Design (RCBD). The results of the study indicated that the interaction of the three factors has a significant effect on the morphological characteristics of the banana plantlets. The average plant height and leaf area increment had a significant impact on the irrigation frequency and the amount of polymer. On the other hand, the girth size was only affected by the amount of irrigation and polymer. Moisture retention was also observed in the treatment with the polymer.